We narrowed to 15,729 results for: grna

1. pLL2 sgRNA(MS2)-MS2-AID3c

   Plasmid

   #112128

   Purpose

   empty sgRNA cloning vector with MS2-AID3c

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono 3c mutant (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA(MS2)_zeo insert c-MYC

   Plasmid

   #164636

   Purpose

   sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

   Depositor

   Günter Schneider

   Insert

   target sequence for c-Myc activation (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS

   Plasmid

   #216830

   Purpose

   cardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   PALMDgRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EGFR-T790M-tmpknot-epegRNA

   Plasmid

   #214093

   Purpose

   Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR T790M epegRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSB-609 U6-sgRNA1 ATCC GGAA

   Plasmid

   #173671

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

   Plasmid

   #118845

   Purpose

   Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   ptena and ptenb (ptena, ptenb Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-pMecp2-dSaCas9-pU6-sgRNA

   Plasmid

   #159174

   Purpose

   Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

   Plasmid

   #163176

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

    Plasmid

    #202822

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs that delete exon 1 within the ZNF91 gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. non-targeting control gRNA (BRDN0001146004)

    Plasmid

    #80174

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKSB-610 U6-sgRNA2 GGAA AGAG

    Plasmid

    #173672

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192682

    Purpose

    Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro U6 sgRNA SOX17 -177

    Plasmid

    #50925

    Purpose

    U6 driven sgRNA targeting Sox17 -177 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Sox17 -177 sgRNA (SOX17 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. non-targeting control gRNA (BRDN0001149423)

    Plasmid

    #80255

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

    Plasmid

    #107857

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 1) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

    Plasmid

    #194015

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.1)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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