We narrowed to 9,135 results for: Pol

1. pGL3-OF (mutant TCF-4 sites)

   Plasmid

   #16559

   Depositor

   Bert Vogelstein

   Article

   Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

   Insert

   TCF-4 binding sites (TCF4 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   3 copies of mutant Tcf-4 binding sites

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTon1(acgfp1)

   Plasmid

   #233648

   Purpose

   Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

   Depositor

   Takeo Kubo

   Article

   Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

   Insert

   target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
   

   Use

   Amphibian expression, tet-on

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX Flag RagC S75N

   Plasmid

   #112757

   Purpose

   express Flag-RagC (S75N mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (S75N) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   S75N

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsp70: mKate2-DN Kif5a

   Plasmid

   #105972

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Kif5a (kif5aa Zebrafish)
   

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   truncation of aa416-1033

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET14b-dabAB2

   Plasmid

   #133013

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO-mCherry GFP

   Plasmid

   #78535

   Purpose

   Control plasmid (paired gRNAs against GFP)

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVETL-Cmcs-Flag-Ataxin3Q22-C14A

   Plasmid

   #22119

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C14A

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

10. cyclin D1-HA-pBabe-Hygro

    Plasmid

    #22703

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. -227 hTF (mutant AP-1)

    Plasmid

    #15444

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Both AP-1 sites mutated

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pVETL-Cmcs-Flag-Ataxin3Q80-C14A

    Plasmid

    #22121

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C14A

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX Flag RagC W115R

    Plasmid

    #112759

    Purpose

    express Flag-RagC (W115R mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (W115R) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    W115R

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CIBN-GFP-TMD

    Plasmid

    #79571

    Purpose

    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    syntaxin 1A (Stx1a Mouse)
    

    Tags

    CIBN and EGFP

    Expression

    Mammalian

    Mutation

    aa 249_288 (C-terminal region (polybasic stretch …

    Promoter

    CMV

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX Flag RagC T90N

    Plasmid

    #112758

    Purpose

    express Flag-RagC (T90N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (T90N) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    T90N

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLyGo-Ec-7

    Plasmid

    #163135

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Nanobody, C-IgAP autotransporter

    Expression

    Bacterial

    Promoter

    P(rhaB)

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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