We narrowed to 13,747 results for: Cre

Showing: 5441 - 5460 of 13747 results

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pET28a T7pCONS sfGFP

Plasmid

#154462

Purpose

pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Publication

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Publication

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-PLlambda-alkS(WT)

Plasmid

#44382

Depositor

Hal Alper

Publication

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkS(WT)

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlambda

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170995

Purpose

HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

Depositor

Alice Ting

Publication

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_3xflag_NRF2(RR)

Plasmid

#136535

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

Depositor

Kevin Janes

Publication

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Mutation

This plasmid is developed by mutating 3 base pair…

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-MYO5C

Plasmid

#135404

Purpose

Expresses GFP-tagged human MYO5C in mammalian cells.

Depositor

Richard Cheney

Publication

Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

Insert

MYO5C (MYO5C Human)

Tags

EGFP

Expression

Mammalian

Mutation

Relative to the GenBank MYO5C cDNA sequence repor…

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDualEX-SMN2

Plasmid

#214431

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Publication

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-Zeo (pRW1445)

Plasmid

#216218

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance

Depositor

Paul Blainey

Publication

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRi(v1)-Blast

Plasmid

#170067

Purpose

Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

Depositor

Neville Sanjana

Publication

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmSfrp2

Plasmid

#31249

Depositor

Jeremy Nathans

Publication

Rattner et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

Insert

Sfrp2 (Sfrp2 Mouse)

Use

Cdna for in situ hybridization

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-EGFAB(H306Y)

Plasmid

#25771

Depositor

Johann Deisenhofer

Publication

McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

Insert

LDL receptor EGFAB (LDLR Human)

Tags

GST

Expression

Bacterial

Mutation

His 306 to Tyr

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
GIPR-DuET

Plasmid

#213252

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GIPR (GIPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Publication

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a T7pCONS TIR-1 sfGFP

Plasmid

#154463

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Publication

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKSII/INSeGFP

Plasmid

#53787

Purpose

1 kb zebrafish insulin promoter driving eGFP

Depositor

Larry Moss

Publication

diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Insert

INS promoter/eGFP/pA

Use

Zebrafish

Tags

eGFP

Expression

Bacterial

Promoter

zebrafish insulin/ZIP

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only
pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EF1a_OTX2_P2A_Hygro_Barcode

Plasmid

#120471

Purpose

Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

OTX2 (OTX2 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC5

Plasmid

#118863

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

Depositor

Marc Bickle, PEPC MPI CBG

Publication

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ERG_P2A_Hygro_Barcode

Plasmid

#120434

Purpose

Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ERG (ERG Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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