We narrowed to 15,732 results for: LEA

1. GFP-p38alpha

   Plasmid

   #86832

   Purpose

   mammalian expression of GFP-p38alpha

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   p38alpha (MAPK14 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-GFP-P2A-MCS (Neo)

   Plasmid

   #89181

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-HRV3C-AcrIF9

   Plasmid

   #141442

   Purpose

   Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

   Depositor

   Blake Wiedenheft

   Article

   Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

   Insert

   AcrIF9
   

   Tags

   HRV3C cleavage site and Maltose Binding Protein

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

   Plasmid

   #205316

   Purpose

   All-in-one AAV vector expressing ISAam1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISAam1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

   Plasmid

   #176541

   Purpose

   human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   OgeuIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ FLAG-hPar1

   Plasmid

   #53226

   Purpose

   mammalian expression of human Par1 with N-terminal FLAG tag

   Depositor

   Shaun Coughlin

   Article

   Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

   Insert

   Par1 (F2R Human)
   

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-39 (native signal peptide)

   Promoter

   SV40

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY001 (pFnCpf1_full)

   Plasmid

   #69973

   Purpose

   Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. plentiCMV-sct-p5-b2m-HLA-A0101

    Plasmid

    #196512

    Purpose

    Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a CMV peptide, b2m and HLA A0101
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human_TFAM_NoMTS_pET28

    Plasmid

    #34705

    Depositor

    David Chan

    Article

    Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

    Insert

    TFAM (TFAM Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Encodes residues 43–246, corresponding to full-le…

    Promoter

    T7

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-PKM2-WT

    Plasmid

    #122690

    Purpose

    Express His-tagged PKM2 (wild type) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA1 Flag ATXN1[85Q]

    Plasmid

    #33237

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 85 Glutamines and no…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAdSh.PGK.Cas9

    Plasmid

    #58253

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    Human codon-optimized S. pyogenes Cas9
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. VP16-ER alpha

    Plasmid

    #11351

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER alpha (ESR1 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. SiC-V2-Cas9G7

    Plasmid

    #133043

    Purpose

    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    Tet repressor
    

    Use

    Lentiviral

    Tags

    Cerulean

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Delta(Pro-MP)ADAM17-HA

    Plasmid

    #65221

    Purpose

    Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM17 (ADAM17 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 18-473

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgPBRM1-1- LentiCRISPRv2

    Plasmid

    #107403

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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