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pCAGGS_3E5E_eGFP

Plasmid

#103054

Purpose

Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

Ebola virus (ebolavirus Zaire, Mayinga) minigenome

Tags

3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-103)

Plasmid

#240597

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-103 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRK172 asyn(1-122)

Plasmid

#240598

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

Ni et al J Mol Biol. 2019 Sep 6;431(19):3913-3919

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

aa 1-122 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNG1256

Plasmid

#149710

Purpose

Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

rpoZ (rpoZ )

Expression

Bacterial

Promoter

T7

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagA

Plasmid

#112740

Purpose

express FRB-myc-RagA

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagA (RRAGA Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagC

Plasmid

#112741

Purpose

express FRB-myc-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagC (RRAGC Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCWXPG-UBI-MafA

Plasmid

#114302

Purpose

Constitutive expression of the MafA trancription factor

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

MafA (MAFA Human)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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iNos-eGFP

Plasmid

#207251

Purpose

Reporter for expression of eGFP under control of iNos promoter

Depositor

Michelle Farkas

Article

Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

Insert

iNos Promoter (Nos2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS_NP_EBOV

Plasmid

#103049

Purpose

Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)

Expression

Mammalian

Mutation

silent mutation (T to C) at position 928 of NP ge…

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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