We narrowed to 9,167 results for: Pol

1. mActA-mSspB-mCherry

   Plasmid

   #210294

   Purpose

   ActuAtor for iLID optogenetics

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   mActA-,SspB-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4_EGR1p-BC1484-luc2

   Plasmid

   #227138

   Purpose

   Barcoded assay, MAPK sensor; barcode BC1484

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   EGR1 promoter driving barcode 1484 and a luciferase reporter gene
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4_EGR1p-BC0136-luc2

   Plasmid

   #227137

   Purpose

   Barcoded assay, MAPK sensor; barcode BC0136

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   EGR1 promoter driving barcode 0136 and a luciferase reporter gene
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4_EGR1p-BC0132-luc2

   Plasmid

   #227136

   Purpose

   Barcoded assay, MAPK sensor; barcode BC0132

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   EGR1 promoter driving barcode 0132 and a luciferase reporter gene
   

   Expression

   Mammalian

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. px552-sg-gria1-HT

   Plasmid

   #187652

   Purpose

   Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Inserts

   HaloTag donor sequence
   miRFP670
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-EF1a-pA (JDW 1222)

   Plasmid

   #224526

   Purpose

   A Gateway compatible 3' entry clone containing rat EF-1a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EF-1a polyA
   

   Use

   Gateway cloning

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYAMTr2GCsgSc-SeLEU2

   Plasmid

   #224871

   Purpose

   Disruption of LEU2 genes in the Saccharomyces sense strict group species

   Depositor

   Katsunori Suzuki

   Article

   Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

   Insert

   pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

   Plasmid

   #222107

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-mCherry

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid

    #169133

    Purpose

    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

    Plasmid

    #222108

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-GFP

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-PODXL S481D

    Plasmid

    #195224

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Expression

    Mammalian

    Mutation

    S481D

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

    Plasmid

    #221082

    Purpose

    Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2
    

    Use

    Synthetic Biology

    Promoter

    CMV, T7

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPicZ:mTREK-1_cryst_I110D

    Plasmid

    #209640

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
    

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA269

    Plasmid

    #216073

    Purpose

    Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

    Plasmid

    #212809

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

    Plasmid

    #212804

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-P2A-3xFLAG-Ubc7
    

    Expression

    Mammalian

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

    Plasmid

    #212803

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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