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pFA197

Plasmid

#149602

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

Depositor

Michael Jensen

Publication

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

PcaO_1O8

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEDJ-22

Plasmid

#140904

Purpose

MarkerFree plasmid for integration of pADH1-PCP-Mxi1 into site X-4

Depositor

Michael Jensen

Publication

Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

Insert

PCP-Mxi1

Promoter

ADH1

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMM756

Plasmid

#133600

Purpose

Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Publication

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG25

Use

Synthetic Biology

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM755

Plasmid

#133599

Purpose

Part Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Publication

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG11

Use

Synthetic Biology

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM764

Plasmid

#133601

Purpose

Part Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Publication

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgTEF1

Use

Synthetic Biology

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEMY07AB-PRO

Plasmid

#127710

Purpose

Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRPB1–4stop

Plasmid

#112824

Purpose

pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

pSMF2 with repeats 8–10 interrupted with 4 repeat…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_chr4_0883tt

Plasmid

#98524

Purpose

BB1 with insert: chr4_0883tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

chr4_0883tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_IDP1tt

Plasmid

#98525

Purpose

BB1 with insert: IDP1tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

IDP1tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS3tt

Plasmid

#98527

Purpose

BB1 with insert: RPS3tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS3tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPL2Att

Plasmid

#98528

Purpose

BB1 with insert: RPL2Att

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPL2Att

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_TDH3tt

Plasmid

#98520

Purpose

BB1 with insert: TDH3tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

TDH3tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS2tt

Plasmid

#98521

Purpose

BB1 with insert: RPS2tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS2tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPP1Btt

Plasmid

#98522

Purpose

BB1 with insert: RPP1Btt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPP1Btt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS17Btt

Plasmid

#98523

Purpose

BB1 with insert: RPS17Btt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS17Btt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFS345

Plasmid

#89349

Purpose

Beetle Luciferase C-term tagging vector

Depositor

Nick Rhind

Publication

Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

Insert

Beetle luciferase

Use

C-terminal tagging vector

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pFS346

Plasmid

#89350

Purpose

Renilla Luciferase C-term tagging vector

Depositor

Nick Rhind

Publication

Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

Insert

Renilla luciferase

Use

C-terminal tagging vector

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Publication

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4-Bre1

Plasmid

#73617

Purpose

Control of the E3 ligase Bre1 with LINXa in yeast.

Depositor

Brian Kuhlman

Publication

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa4-Bre1dNLS

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

Plasmid

#203657

Purpose

Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

GFPMUT3

Expression

Yeast

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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