Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites.
Babaei M, Sartori L, Karpukhin A, Abashkin D, Matrosova E, Borodina I
FEMS Yeast Res. 2021 May 11;21(4). pii: 6249452. doi: 10.1093/femsyr/foab027. PubMed Article
Babaei M, Sartori L, Karpukhin A, Abashkin D, Matrosova E, Borodina I
FEMS Yeast Res. 2021 May 11;21(4). pii: 6249452. doi: 10.1093/femsyr/foab027. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161589 | pCfB9336 (pgRNA_II-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1 |
| 161590 | pCfB9337 (pgRNA_IV-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1 |
| 161591 | pCfB9339 (pgRNA_VII-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1 |
| 161592 | pCfB9340 (pgRNA_VIII-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1 |
| 161593 | pCfB9341 (pgRNA_IX-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1 |
| 161594 | pCfB9342 (pgRNA_XIII-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1 |
| 161595 | pCfB9343 (pgRNA_XV-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1 |
| 161596 | pCfB9344 (pgRNA_XVI-1_NatMX) | EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1 |
| 161597 | pCfB9355(II-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641) |
| 161598 | pCfB9356(IV-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940) |
| 161599 | pCfB9358(VII-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490) |
| 161600 | pCfB9359(VIII-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634) |
| 161601 | pCfB9360(IX-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883) |
| 161602 | pCfB9361(XIII-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823) |
| 161603 | pCfB9362(XV-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205) |
| 161604 | pCfB9363(XVI-1_Markerfree_BackBone) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286) |