We narrowed to 6,084 results for: nls

1. AA122

   Plasmid

   #215980

   Purpose

   Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA091

   Plasmid

   #215979

   Purpose

   Fragmid fragment: (N' terminus) ZNF10 KRAB

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; KRAB_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA088

   Plasmid

   #215978

   Purpose

   Fragmid fragment: (N' terminus) ZIM3 KRAB

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; KRAB_v2.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA095

   Plasmid

   #216043

   Purpose

   Fragmid fragment: (C' terminus) ZNF10 KRAB

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NpNLS_v1.1; KRAB_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA012

   Plasmid

   #215974

   Purpose

   Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA370

   Plasmid

   #215988

   Purpose

   Fragmid fragment: (N' terminus) attenuate expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Linked mRuby3 VLP

   Plasmid

   #182430

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-GFP-mRuby3
   deltaVP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Coexpressed mRuby3 VLP

   Plasmid

   #182431

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-GFP
   deltaVP1
   VP2C-mRuby3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHES 946

   Plasmid

   #112644

   Purpose

   blue light inducible cryptochrome based optogenetic expression system

   Depositor

   Hana El-Samad

   Article

   Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

   Insert

   RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

    Plasmid

    #233078

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

    Plasmid

    #233080

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1i

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

    Plasmid

    #233079

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1a

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EFS-CjCas9-eGFP-HIF1a

    Plasmid

    #137929

    Purpose

    Expression of cjCas9 with Hif1a targeting sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40NLS-HA-T2A-EGFP

    Expression

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. rnLbCas12a-PE2

    Plasmid

    #213746

    Purpose

    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    rnLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, H759A, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78304

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCL.175

    Plasmid

    #184996

    Purpose

    Express -Eco1 HEK3 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK3 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL.177

    Plasmid

    #184998

    Purpose

    Express -Eco1 EMX1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    EMX1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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