We narrowed to 9,167 results for: Pol

1. pcDNA5FRT/TO-GFP-I3(resist)

   Plasmid

   #61400

   Purpose

   Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   silent mutations of bases C183T T192C T189C

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5FRT/TO-GFP-I3(V41A/W43A)

   Plasmid

   #61402

   Purpose

   Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   inhibitor-3 (PPP1R11 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   V41A/W43A

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. psicheck-2-Mc2r

   Plasmid

   #48174

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of MC2R (MC2R Human)
   

   Use

   Luciferase

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pFM038

   Plasmid

   #40141

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R183-5A + 7SA; base pairs 6-523 of par-2 were re…

   Promoter

   pie-1

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pFM041

   Plasmid

   #40144

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   S241A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pFM039

   Plasmid

   #40142

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R183-5A + S241A; base pairs 6-523 of par-2 were …

   Promoter

   pie-1

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pFM036

   Plasmid

   #40139

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (K162A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   K162A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFM052

   Plasmid

   #40146

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (C56S) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   C56S; base pairs 6-523 of par-2 were recoded to …

   Promoter

   pie-1

   Available Since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFM037

   Plasmid

   #40140

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R163A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R163A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1994

    Plasmid

    #49124

    Purpose

    AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple266-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    S100B

    Available Since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. hGFAP-GFP

    Plasmid

    #40592

    Depositor

    Albee Messing

    Article

    Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

    Insert

    GFP-S65T
    

    Use

    Mouse Targeting

    Tags

    MP-1 fragment (mouse protamine 1 intron and polya…

    Expression

    Bacterial and Mammalian

    Mutation

    Construct contains a mutated, humanized hGFP(S65T…

    Promoter

    hGFAP promoter

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4_NFAT-RE-CMVmin-BC0524-luc2

    Plasmid

    #227139

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC0524

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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