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Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK-Cas9-gate
Plasmid#113743PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorTagsExpressionMutationPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-130
Plasmid#118548PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LB901: pMVP/PB/Blast-DEST
Plasmid#121873PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-165
Plasmid#118577PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-167
Plasmid#118579PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35S-GW-3Avi
Plasmid#127918PurposeGateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplastDepositorTypeEmpty backboneUseTags3xAviTagExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-163
Plasmid#118575PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ210
Plasmid#109329PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selectionDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
JN302: pMVP/SB/Puro-DEST
Plasmid#121865PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-152
Plasmid#118534PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-147
Plasmid#118563PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB701: pMVP/PB/mCherry-DEST
Plasmid#121871PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-N-FLAG
Plasmid#79549PurposeDestination vector containing an N-terminal FLAG tagDepositorInsertFLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE-Tn5-133
Plasmid#118519PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-135
Plasmid#118521PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only