We narrowed to 16 results for: chloramphenicol
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TypeBlog Post...General protein expression BL21 (DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce...Expression of toxic proteins BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression...Expression of insoluble proteins Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal” translation; contains...Expression of eukaryotic proteins Lemo21 (DE3)* Chloramphenicol (pLemo) Rhamnose-tunable T7 RNAP expression...T7 expression. The pLys plasmid contains a chloramphenicol resistance cassette for positive selection ...
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Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol Binds 50S ribosomal subunit; inhibits peptidyl... -
Plasmids 101: Origin of Replication
TypeBlog Post...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working... -
Working with Nuclear Receptors
TypeBlog Post...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ... -
CRISPR Plasmids - Bacteria
TypeCollection...Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria BBa_J23119... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...hsdR17(rK- mK+) XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of ... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting... -
Pouring LB Agar Plates
TypeProtocol...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin... -
Sequencing Primers
TypeGuide...primer CAT-R GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer CMV Forward... -
Molecular Biology Reference
TypeGuide...100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Hygromycin...