We narrowed to 59,355 results for: cloning

1. pME-NR2F2

   Plasmid

   #138359

   Purpose

   Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   Human NR2F2
   

   Expression

   Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KRASB

   Plasmid

   #25153

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   KRASb:M1-K169 (KRAS Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Q61H

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. CMV-HA-hTAF6

   Plasmid

   #17988

   Depositor

   Naoko Tanese

   Article

   Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

   Insert

   TAF6 (TAF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pKO (Apr)

   Plasmid

   #110088

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCZGY2850

   Plasmid

   #87758

   Purpose

   Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pcol-19-PH-miniSOG(Q103L)
   

   Tags

   PH

   Expression

   Worm

   Mutation

   Q103L

   Promoter

   Pcol-19

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO V5 HSPA6

   Plasmid

   #19513

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 6 (HSPA6 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-secAnxaV-NS

   Plasmid

   #67718

   Purpose

   Gateway entry clone containing secreted human AnxaV with no stop codon

   Depositor

   Rob Parton

   Article

   Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

   Insert

   secAnxaV-NS
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-GFP-mCherry-3pGW

   Plasmid

   #53965

   Purpose

   Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

   Inserts

   GFP
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/Myc-His(-)-MSulf-1

   Plasmid

   #13007

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 1 (Sulf1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pDD317 (Pmyo-2::GFP + SEC)

    Plasmid

    #73344

    Purpose

    SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

    Insert

    Pmyo-2::GFP + SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Promoter

    myo-2

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKO (Hyg)

    Plasmid

    #110087

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Promoter

    Ptet

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-IRES2-GFP

    Plasmid

    #234875

    Purpose

    Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSYn

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGS-21a-PcAad1

    Plasmid

    #163039

    Purpose

    expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

    Depositor

    Jean-Marie Francois

    Article

    Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

    Insert

    aryl-alcohol dehydrogenase
    

    Tags

    GST and His6

    Expression

    Bacterial

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCPP5741

    Plasmid

    #128722

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcV-hopV1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid

    #189871

    Purpose

    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPP5910

    Plasmid

    #128717

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcO-hopO1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP5058

    Plasmid

    #128710

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcF-hopF2
    

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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