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pTwist-WIV1-CoV Δ18

Plasmid

#164439

Purpose

Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike truncated to remove 18 amino acids

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-Switch

Plasmid

#99733

Purpose

Flp-based FLEX reporter (from RFP to GFP)

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

TagRFP and EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF1-V2

Plasmid

#69045

Purpose

expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

splice variant 2

Promoter

IRES

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK3 Q233D

Plasmid

#80874

Purpose

mammalian expression of ALK3 Q233D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK3 (BMPR1A Human)

Tags

HA

Expression

Mammalian

Mutation

Q233D (constitutively active)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
Tol2 8xSTAR-mNeonGreen. PGK-puro

Plasmid

#136255

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-αIFNα-ib

Plasmid

#110798

Purpose

Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

Depositor

Thomas Böldicke

Article

Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

Insert

intrabody against mouse interferon alpha

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

Plasmid

#78099

Purpose

Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

Histone H2B - Citrine (H2BC21 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

5xTetO pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

Plasmid

#78349

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 1

Plasmid

#22209

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A1_GTP-EFTU

Plasmid

#109861

Purpose

Protein expression and purification of EEF1A1_GTP-EFTU

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A1_GTP-EFTU (EEF1A1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FRIG-myc-Sema4D-CD4

Plasmid

#51606

Purpose

expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Use

Lentiviral

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
TV-hPITX3-forward

Plasmid

#22073

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3 (ASMD Human)

Use

Cre/Lox; Zfn donor

Tags

eGFP

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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