We narrowed to 15,972 results for: grna

1. lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry

   Plasmid

   #202821

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (two)

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

   Plasmid

   #202825

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSK-mU6-Ccu-sgRNA-SV40-puro

   Plasmid

   #192130

   Purpose

   Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Ccu-SgRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSK-mU6-Hsp1-sgRNA-SV40-puro

   Plasmid

   #192129

   Purpose

   Expresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-SgRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

   Plasmid

   #194725

   Purpose

   based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D908A

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

   Plasmid

   #188705

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   ST sgRNAs
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGW02 AAV-TBG-Cre-sgP53-sgRNA

   Plasmid

   #192162

   Purpose

   AAV-TBG-Cre-sgP53-sgRNA

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   AAV

   Mutation

   NA

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

   Plasmid

   #167921

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB700 lenti gRNA Blasto_TtoC and hairpin extension

    Plasmid

    #167920

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB700 lenti gRNA EYFP_TtoC and hairpin extension

    Plasmid

    #167922

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB700 lenti gRNA Hygro_TtoC and hairpin extension

    Plasmid

    #167923

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

    Plasmid

    #167924

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB700 lenti gRNA Puro_TtoC and hairpin extension

    Plasmid

    #167927

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

    Plasmid

    #170792

    Purpose

    This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNTI743 dual-barcoded gRNA parent vector

    Plasmid

    #164915

    Purpose

    CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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