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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pRKVSV FAT

Plasmid

#50533

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
CLM RP KGF Mut 1

Plasmid

#24166

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pKlab1197-pcDNA-3.1-3xFLAG-V5-hPALB2 (T333A, L703L, H1184R)

Plasmid

#249003

Purpose

This plasmid expresses the hPALB2 protein seqence for pull down assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

hPALB2 (PALB2 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSA081 BFP

Plasmid

#249138

Purpose

Overexpression of BFP. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

TagBFP

Use

Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1583_ECOR31HamA(E138AK140A)B_CoPurification

Plasmid

#223372

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)

Tags

MBP-His-TEV

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-WT

Plasmid

#248131

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2)

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2 (Vamp2 Rat)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-0-smGFP-HA

Plasmid

#247945

Purpose

Conditional tagging of endogenous proteins with smGFP-HA in open reading frame 0, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

smGFP-HA

Tags

smGFP-HA

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-1-smGFP-HA

Plasmid

#247946

Purpose

Conditional tagging of endogenous proteins with smGFP-HA in open reading frame 1, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

smGFP-HA

Tags

smGFP-HA

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-2-smGFP-HA

Plasmid

#247948

Purpose

Conditional tagging of endogenous proteins with smGFP-HA in open reading frame 2, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

smGFP-HA

Tags

smGFP-HA

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget-entry

Plasmid

#220996

Purpose

Prokaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

mRFP, GFP

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2

Plasmid

#236733

Purpose

Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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