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We narrowed to 826 results for: Cerulean

Showing: 581 - 600 of 826 results
  1. pcDNA3-ER-AKAR4

    Plasmid
    #64733
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
    Depositor
    Insert
    AKAR4
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Insert
    Syntaxin 1A (Stx1a Rat)
    Use
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1(+)-Mito-CaNARi

    Plasmid
    #64851
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Insert
    CaNARi
    Use
    Tags
    Cerulean, N-terminal 30 amino acids from DAKAP1, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1(+)-ER-CaNARi

    Plasmid
    #64852
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
    Depositor
    Insert
    CYP450-CaNARi
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Insert
    Mesh1 (Mesh1 Fly)
    Use
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    AMPKsub-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    PKCsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Insert
    PKAsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Insert
    JNKsubDD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Insert
    PKAsub-YPet-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
  12. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 581 - 600 of 826 results