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pAAV.RMacL5ET-06.EGFP.BCB4.WPRE

Plasmid

#229632

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-06.EGFP.BCB4

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-09.EGFP.BCB8.WPRE

Plasmid

#229635

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-09.EGFP.BCB8

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-10.EGFP.BCC3.WPRE

Plasmid

#229636

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-10.EGFP.BCC3

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-11.EGFP.BCC4.WPRE

Plasmid

#229637

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-11.EGFP.BCC4

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-04.EGFP.BCB1.WPRE

Plasmid

#225688

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-04.EGFP.BCB1

Expression

Mammalian

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-02.EGFP.BCA7.WPRE

Plasmid

#225686

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-02.EGFP.BCA7

Expression

Mammalian

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-03.EGFP.BCA12.WPRE

Plasmid

#225687

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-03.EGFP.BCA12

Expression

Mammalian

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1093-luc2

Plasmid

#227113

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

Depositor

Michael Wehr

Publication

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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1533_pAAV-CB-AcGFP-BirA-HA

Plasmid

#208201

Purpose

Control for FKBP12-BirA construct

Depositor

Susan Hamilton

Publication

Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

Type

Empty backbone

Use

AAV

Promoter

Chicken beta actin

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgIdua.CMV/CB-EGFP

Plasmid

#121511

Purpose

Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgIdua

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1049-pAAV-mscRE13-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163482

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1049 - pAAV-AiE2013m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Igk-HT9-PDGFR-IRES-sfGFP-WPRE

Plasmid

#252088

Purpose

Expresses membrane bound halotag in mammalian cells with GFP fluorophore

Depositor

Karl Deisseroth

Publication

Zhao et al Nat Chem. 2026 Jul 8. doi: 10.1038/s4155

Insert

Igk-HT9-PDGFR-IRES-sfGFP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Igk-HT9-PDGFR-IRES-BFP-WPRE

Plasmid

#252086

Purpose

Expresses membrane bound halotag in mammalian cells with BFP fluorophore

Depositor

Karl Deisseroth

Publication

Zhao et al Nat Chem. 2026 Jul 8. doi: 10.1038/s4155

Insert

Igk-HT9-PDGFR-IRES-BFP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-206-MRE

Plasmid

#235156

Purpose

Expresses the mCherry-based sensor for miR-206. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Publication

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a miR-206 MRE from Tpm4

Use

AAV

Promoter

EFS

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Flex-Kir2.2mut-t2a-mRuby3

Plasmid

#255749

Purpose

Expresses a mutated Kir2.2 potassium channel and a red fluorescent marker in a cre dependent manner

Depositor

Ivo Spiegel

Publication

Shi et al Neuron. 2026 Apr 2:S0896-6273(26)00180-7

Inserts

KCNJ12 (Kcnj12 Mouse)
mRuby

Use

AAV

Mutation

GYG to AAA (aa145-147)

Promoter

hSyn

Available since

July 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Flex-Kir2.2wt-t2a-mRuby3

Plasmid

#255748

Purpose

Expresses a wt Kir2.2 potassium channel and a red fluorescent marker in a cre dependent manner

Depositor

Ivo Spiegel

Publication

Shi et al Neuron. 2026 Apr 2:S0896-6273(26)00180-7

Inserts

KCNJ12 (Kcnj12 Mouse)
mRuby

Use

AAV

Promoter

hSyn

Available since

July 29, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA

Plasmid

#192485

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Publication

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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