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pMC-6-ZmBbm-9

Plasmid

#197748

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmBbm

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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ChlorON-2

Plasmid

#193478

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-2

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_mMSH2

Plasmid

#186156

Purpose

sgRNA targeting murine Msh2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

Msh2_gRNA (Msh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-610 U6-sgRNA2 GGAA AGAG

Plasmid

#173672

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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PX458-gR2A_ISO

Plasmid

#124808

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

hSPCas9

Tags

3XFLAG and GFP

Expression

Mammalian

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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BSP602

Plasmid

#122249

Purpose

expression vector for mScarlet under EFT3 Promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mScarlet

Use

Synthetic Biology

Expression

Worm

Promoter

EFT3

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Expression

Yeast

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pDM1210

Plasmid

#108987

Purpose

extrachromosomal expression vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRVV669

Plasmid

#108283

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgAY-CmR

Plasmid

#98938

Purpose

Conductive Curli Protein csgAY

Depositor

Urartu Seker

Article

Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

Insert

csgAY

Tags

YYRKYKEYDDY

Expression

Bacterial

Promoter

tetO

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C3106

Plasmid

#91101

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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mPapaya-CytERM-N-17

Plasmid

#56345

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CytERM

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-PUb-SSA

Plasmid

#52910

Purpose

Ae aegypti PUb promoter Single Stranded Annealing reporter

Depositor

Zach Adelman

Article

Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

Insert

Luciferase duplication-TALEN recognition sites

Use

Luciferase

Promoter

Ae aegypti polyubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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