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EF1a-Flex-MCS-WPRE-SV40 - GCH14

Plasmid

#230821

Purpose

AAV cloning vector for CRE-dependent expression under the EF1a promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV and Cre/Lox; Cloning vector

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mitoEmGFP

Plasmid

#240414

Purpose

Cre-dependent expression of mito-EmGFP

Depositor

Oscar Marin

Publication

Moissidis et al Cell. 2025 Oct 2;188(20):5555-5575.e26.

Insert

Mito

Use

AAV and Cre/Lox

Tags

EmGFP

Expression

Mammalian

Promoter

hSyn

Available since

Sept. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Ppargc1a1

Plasmid

#240415

Purpose

Cre-dependent expression of the long canonical isoform of Ppargc1a (Ppargc1a1)

Depositor

Oscar Marin

Publication

Moissidis et al Cell. 2025 Oct 2;188(20):5555-5575.e26.

Insert

Ppargc1a long isoform (Ppargc1a )

Use

AAV and Cre/Lox

Tags

Flag and HA

Expression

Mammalian

Promoter

hSyn

Available since

Sept. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244079

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244093

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244098

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244082

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244086

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244080

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.HaloTag

Plasmid

#244085

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

HaloTag

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-01.ChR2(H134R)-GFP.BCC10

Plasmid

#229639

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-01.ChR2(H134R)-GFP.BCC10

Use

AAV

Expression

Mammalian

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT2m

Plasmid

#208716

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)

Plasmid

#214843

Purpose

Expression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

ChR2(L132C, H134R, T159C)-EYFP

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165492

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Publication

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

hSyn1

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CreOff-ChRmine-mScarlet-WPRE

Plasmid

#224137

Purpose

Expresses ChRmine-mScarlet in a CreOff-dependent vector

Depositor

Rafael Yuste

Publication

Cornejo et al Sci Adv. 2026 Aug 14;12(33):eadz4123. do

Insert

ChRmine-mScarlet

Use

AAV

Expression

Mammalian

Available since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

Plasmid

#233270

Purpose

To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

Depositor

Jonathan Ploski, Glenn Schafe

Publication

Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

Insert

GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)

Use

AAV

Tags

GFP

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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