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PACmn for improved optogenetic control of intracellular cAMP.
Yang S, Constantin OM, Sachidanandan D, Hofmann H, Kunz TC, Kozjak-Pavlovic V, Oertner TG, Nagel G, Kittel RJ, Gee CE, Gao S
BMC Biol. 2021 Oct 18;19(1):227. doi: 10.1186/s12915-021-01151-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
165487pGEM-HE-Venus-bPAC(F198Y)Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity
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165488pGEM-HE-bPAC(R278A)-Myc-eYFPXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity
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165489pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
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165490pGEM-HE-Glyco-Venus-bPAC(S27A)Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity
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165491pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .
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165492pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
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165493pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
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171117pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
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