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pKERepPar

Plasmid

#211827

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Publication

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBSPPc

Plasmid

#116918

Purpose

Gene constitutive expression from a Pc promoter

Depositor

Cuiqing Ma

Publication

Xu et al Appl Environ Microbiol. 2013 Apr;79(8):2

Type

Empty backbone

Expression

Bacterial

Promoter

Pc promoter from Delftia acidovorans C17

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLys-M1 backbone

Plasmid

#109386

Purpose

Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

Depositor

Tom Ellis

Publication

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Expression

Bacterial

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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Bsrs078-TraR

Plasmid

#85150

Purpose

Constitutively expresses TraR

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TraR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS383

Plasmid

#73736

Purpose

SapTrap TEV-Flagtag N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

TEV-FLAGtag

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS282

Plasmid

#73733

Purpose

SapTrap FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

N-terminal FLP-on

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Publication

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-400_Cacnes_CAMP2

Plasmid

#225606

Purpose

Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

Depositor

Marc Güell

Publication

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Georg Fritz, Thorsten Mascher

Publication

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAEF5

Plasmid

#136305

Purpose

Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

Depositor

Gilles Fischer

Publication

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Type

Empty backbone

Use

CRISPR

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB2804

Plasmid

#63644

Purpose

pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMGAHisTev5B

Plasmid

#37221

Depositor

Jon Lorsch

Publication

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF5B

Tags

His

Mutation

N-term truncation (contains aa 396-1002)

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_mCherry

Plasmid

#221470

Purpose

Gateway cloning of mCherry overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

mCherry

Use

Gateway Cloning

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGLO-Pink-SHE

Plasmid

#220781

Purpose

Expresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

Depositor

Angela Leu

Insert

raspberry chromoprotein (mRFP1)

Tags

6xHis

Expression

Bacterial

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Publication

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PE_CO-Mini-N

Plasmid

#190336

Purpose

Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Publication

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion

Use

AAV

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pOsU3-esgRNA

Plasmid

#115629

Purpose

expression esgRNA in rice protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

OsU3p-esgRNA

Use

CRISPR

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS281

Plasmid

#73732

Purpose

SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded N-terminal FLP-on

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-p65AD::CaM

Plasmid

#64719

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Expression

Insect

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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