We narrowed to 9,173 results for: Pol

1. pLV-CDH5-mCitrine-IRES-puro

   Plasmid

   #85143

   Purpose

   lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   mCitrine

   Promoter

   CAG

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti.CMV/TO_PRKAA2

   Plasmid

   #74447

   Purpose

   Lentiviral expression vector encoding untagged WT human AMPK alpha 2

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   AMPK alpha 2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

4. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 GfaABC1D hM4D mCherry SV40

   Plasmid

   #92286

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   hM4D (CHRM4 Human)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   GfaABC1D

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTMiniTrex-mCherry-Shark1

   Plasmid

   #233143

   Purpose

   Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark1
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Mutation

   Codon optimized for Trypanosoma cruzi and changed…

   Available Since

   March 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mouse b3 full length

   Plasmid

   #217814

   Purpose

   Full length mouse integrin b3 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB3 (Itgb3 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. hu-TLR4 cDNA wt pDEST40

    Plasmid

    #42646

    Purpose

    destination vector for expression of human TLR4 in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 (TLR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLentiDP1.3

    Plasmid

    #171096

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, cdt1, miniAID, mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiDP1.2

    Plasmid

    #171095

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, Geminin, miniAID, mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1delZn2

    Plasmid

    #173935

    Purpose

    Bacterial expression of PARP1 lacking Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 (V762A)

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Cdt1 human

    Plasmid

    #72681

    Purpose

    mammalian expression of human Cdt1

    Depositor

    Domenico Maiorano

    Article

    Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

    Insert

    Cdt1 (CDT1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pCI-MS2V5-eRF3a F76a

    Plasmid

    #65811

    Purpose

    expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eRF3a (GSPT1 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    F76a

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

    Plasmid

    #216475

    Purpose

    lentiviral dual expression of YAP5SA and eGFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    eGFP
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-His10TEV

    Plasmid

    #159427

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Tags

    TEV-protease-cleavable His10-tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-V5-hspB5

    Plasmid

    #63106

    Purpose

    Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-ART

    Plasmid

    #174810

    Purpose

    Bacterial expression of isolated PARP1 ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-ART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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