We narrowed to 9,173 results for: Pol

1. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

   Plasmid

   #122957

   Purpose

   Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NLB (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xmyc tag

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-mScarlet-hCenpC

   Plasmid

   #171490

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   CenpC (CENPC Human)
   

   Use

   Pgehme

   Tags

   mScarlet

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-Flag-Rheb-4Rs

   Plasmid

   #165022

   Purpose

   expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-4Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   change lysine residues on position 109, 135, 151,…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-Neo-TetO-Cdk12_Untagged

   Plasmid

   #127178

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Promoter

   TetO Promoter

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR8-PHF19

   Plasmid

   #68864

   Purpose

   GW entry vector for PHF19

   Depositor

   Adrian Bracken

   Article

   Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Gateway entry vector

   Promoter

   none

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pBK_Gal4-DBD-GR(NR3C1)

   Plasmid

   #227100

   Purpose

   GR receptor overexpression for luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   GR (NR3C1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK5867

   Plasmid

   #219751

   Purpose

   pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - mcitHispS - tAOX
   

   Expression

   Yeast

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK1508

   Plasmid

   #219750

   Purpose

   pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - npgA - tAOX
   

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-mTurquoise-MLC-IRES-neo

   Plasmid

   #85145

   Purpose

   lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mTurquoise

   Promoter

   EF1a

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX020

    Plasmid

    #167146

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal caffeoylpiruvate hydrolase, nnCPH
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1delCAT

    Plasmid

    #173942

    Purpose

    Bacterial expression of PARP1 lacking CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delCAT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 661-1014

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRE-Lepr_Short-IRES-eGFP

    Plasmid

    #196935

    Purpose

    Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    short isoform, lack of signaling casacade

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_ER-RE_MLPmin_BC1065-luc2

    Plasmid

    #227111

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. W118-1_Flag-hIRAK1

    Plasmid

    #180405

    Purpose

    lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    IRAK1 (IRAK1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1delWGR

    Plasmid

    #173940

    Purpose

    Bacterial expression of PARP1 lacking WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid

    #172473

    Purpose

    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    

    Use

    Piggybac transposon

    Tags

    polybasic and CAAX motif from Kras, tdKatushka2, …

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PARP1-c009

    Plasmid

    #216872

    Purpose

    Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

    Depositor

    Lari Lehtiö

    Article

    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

    Insert

    PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
    

    Expression

    Bacterial

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. hRAD18 dC2-EGFP

    Plasmid

    #68826

    Purpose

    Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

    Depositor

    Satoshi Tateishi

    Article

    Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

    Insert

    human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

    Plasmid

    #122954

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Promoter

    CAG promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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