We narrowed to 9,173 results for: Pol

1. pAAV-UbC-mEmerald Rab7a

   Plasmid

   #115238

   Purpose

   To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

   Depositor

   Pantelis Tsoulfas

   Insert

   Rab7a fused to mEmerald (RAB7A Human)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   7 Amino Acids (21 Nucleotides) between mEmerald a…

   Promoter

   Human UbC

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

   Plasmid

   #63139

   Purpose

   Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

   Depositor

   Titia Sixma

   Article

   Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

   Inserts

   Ring1b (Ring1 Mouse)
   Bmi1 (Bmi1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-109 and Residues 1-159

   Promoter

   tac

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKIV-DCTK75E

   Plasmid

   #126423

   Purpose

   A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed lysine 75 to glutamic acid

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1delZn3

   Plasmid

   #173936

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK3203

   Plasmid

   #219749

   Purpose

   DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - mcitHispS - tSV40
   

   Expression

   Mammalian

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-hsKIF5B(1-560)Rigor-L-2xHA

   Plasmid

   #193718

   Purpose

   Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2281

    Plasmid

    #111902

    Purpose

    ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    AAV

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid

    #221081

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Frt-hspB1

    Plasmid

    #63092

    Purpose

    Expression of HSP1 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET32a-HD39Q

    Plasmid

    #11514

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 39 glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 39 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid

    #69842

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M-S285A-T289A

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse a5 full length

    Plasmid

    #217810

    Purpose

    Full length mouse integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (Itga5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1delZn1

    Plasmid

    #173933

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-HD

    Plasmid

    #174809

    Purpose

    Bacterial expression of isolated PARP1 HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-HD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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