We narrowed to 18,244 results for: gateway

1. pDEST-CMV 3xFLAG-GABARAPL1-GFP

   Plasmid

   #123112

   Purpose

   Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ230-E795L

   Plasmid

   #176890

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   LbCas12a-E795L
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159850

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEG R2-dsRed-L3 #BGV203

   Plasmid

   #48973

   Purpose

   Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   dsRed
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Promoter

   none

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pMXs-GLIS1 (FLJ33315SAAN)

   Plasmid

   #33274

   Purpose

   Retroviral expression of canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1, Gateway casette A (GLIS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

   Plasmid

   #127359

   Purpose

   gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEG R2-iBlast-L3 #BGV204

   Plasmid

   #48982

   Purpose

   Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   blasticidin-S deaminase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBEG R3-miRNA(ccdB)-L4 #BGV228

   Plasmid

   #48953

   Purpose

   To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. p3E-RhoAb-DN

    Plasmid

    #109580

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative RhoAb

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAb (rhoab Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAFW-Ago1

    Plasmid

    #50553

    Purpose

    Expression of FLAG tagged Ago1 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

    Insert

    Argonaute1 (AGO1 Fly)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159854

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ290

    Plasmid

    #129670

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. VanGlow-GL

    Plasmid

    #83342

    Purpose

    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    GFP::Luciferase(nls)

    Expression

    Insect

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 PAK5

    Plasmid

    #60532

    Purpose

    Donor vector for human Pak5

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR1A DHFR.cHSF1

    Plasmid

    #134737

    Purpose

    Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    DHFR

    Mutation

    Deletion of amino acids 186-202

    Promoter

    None

    Available Since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-ING2

    Plasmid

    #109554

    Purpose

    Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TT 3xFLAG-KPNA2

    Plasmid

    #192306

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA2 (KPNA2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-CreLite

    Plasmid

    #131782

    Purpose

    Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    Cre/Lox; Gateway cloning

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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