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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Publication

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585

Plasmid

#174401

Purpose

S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

Depositor

Dennis Ko

Publication

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Publication

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

Plasmid

#136543

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Publication

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Desmocollin1-N-18

Plasmid

#57370

Purpose

Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Desmocollin1 (DSC1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mTagBFP2-MCHR1-N-10

Plasmid

#55313

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MCHR1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Publication

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Available since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc15_524

Plasmid

#49736

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS15_524

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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ERrefGFP1_pCDNA3

Plasmid

#48635

Purpose

Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

Depositor

David Ron

Publication

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERrefGFP1

Tags

FLAG

Expression

Mammalian

Mutation

C147S,C204Q

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-E64D

Plasmid

#29352

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Glu64Asp

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-GUK1

Plasmid

#23380

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GUK1 (GUK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKX

Plasmid

#23383

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKX (PRKX Human)

Use

Gateway Cloning

Mutation

V43A

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DGKK

Plasmid

#23487

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKK (DGKK Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-PIP5KL1

Plasmid

#23631

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5KL1 (PIP5KL1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CAMKV

Plasmid

#23756

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMKV (CAMKV Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSuper.mBeta826

Plasmid

#12549

Depositor

Robert Sobol

Publication

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

shRNA to mouse polymerase beta

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pSKB3.MPN348

Plasmid

#11437

Depositor

Sung-Hou Kim

Publication

Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

Insert

methenyltetrahydrofolate synthetase

Tags

6x His and TEV cleavage site

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-Lck-mCherry-ibARK-4x6T

Plasmid

#241243

Purpose

Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

DIO-Lck-mCherry-ibARK-4x6T

Use

AAV

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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