We narrowed to 11,809 results for: emb
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Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K29,30A)-mVenus
Plasmid#168485PurposeMammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertthe K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP3FBAR-EGFP
Plasmid#168506PurposeExpression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSRGAP3-FBAR (SRGAP3 Human)
TagsEGFPExpressionMammalianMutationaa 1-492PromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 nickase D10A Cas9 (GB1690)
Plasmid#160587PurposeNickase D10A Cas9 for Nt and Ct fusion.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnickase D10A Cas9
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9wt)-Rif1
Plasmid#122306PurposeExpresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA for mouse Rif1 (Rif1 Synthetic)
ExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(K271Q)
Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsHAExpressionMammalian and YeastMutationK271QAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187
Plasmid#139309PurposeN-terminal half of split PI-PLC targeted to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
ExpressionMammalianAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
FB003
Plasmid#119677PurposeTranscriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic BiologyAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GSO2/EDA23 X079_pECIA14
Plasmid#114911PurposePrey vector GSO2/EDA23 X079_pECIA14 should be used with bait vector GSO2/EDA23 X079_pECIA2.DepositorAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SKM2 X057_pECIA2
Plasmid#115114PurposeBait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.DepositorInsertAT5G56040 (AT5G56040 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SKM1 nA12_pECIA2
Plasmid#115022PurposeBait vector SKM1 nA12_pECIA2 should be used with prey vector SKM1 nA12_pECIA14.DepositorInsertAT2G25790 (AT2G25790 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK2 P5_pECIA2
Plasmid#114968PurposeBait vector SERK2 P5_pECIA2 should be used with prey vector SERK2 P5_pECIA14.DepositorInsertAT1G34210 (SERK2 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTmut full-length
Plasmid#109162PurposeEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression systemDepositorAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PARP14 (PARP14A-c013)
Plasmid#110254PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3HCA8
Plasmid#103100PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D6GRK4
Plasmid#103097PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD6GRK4(Cas9 coding gene from Filifactor alocis (strain ATCC 35896 / D40 B5))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialPromoternative promoter of sll0617Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO
Plasmid#73448PurposeExpress mouse OC-STAMP in mammalian cellsDepositorAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only