Addgene

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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arrSa

Plasmid

#78169

Purpose

zf arrSa in pCRII-TOPO

Depositor

Stephan Neuhauss

Article

Renninger et al Eur J Neurosci. 2011 Feb;33(4):658-67. d

Insert

arrSa

Use

Tags

Expression

Mutation

Promoter

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28b-zDjCas13d-His

Plasmid

#234481

Purpose

Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DjCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-Tol2-PA2-CMV-AB-mCh

Plasmid

#160435

Purpose

Expresses human Amyloid Beta-mCherry in Zebrafish

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)

Use

Zebrafish expression

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2-10xUAS-Voltron

Plasmid

#119039

Purpose

UAS-driven expresion of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

E1b

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-Voltron

Plasmid

#119037

Purpose

Pan neuronal expression of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

elavl3

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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PCRII apoEb

Plasmid

#121901

Purpose

Plasmid for making zebrafish apoEb in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoeb (apoeb Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Expression

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PCRII apoBa

Plasmid

#121897

Purpose

Plasmid for making zebrafish apoBa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBa (apoba Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Article

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Tags

Expression

Mutation

Codon Optimized

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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ZP46 Prickle-2

Plasmid

#16896

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-2 (prickle2b Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
mpeg:lamp1b-mCherry-stop; cmlc2:GFP

Plasmid

#213739

Purpose

Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp1b-mCherry

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Plasmid

#209282

Purpose

Expresses FR-GECO1c and CoChR in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

zACNE20_myl7

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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5UAS zfSynaptophysin:GFP

Plasmid

#74316

Purpose

A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

Depositor

Stephen Smith, Martin Meyer

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Insert

synaptophysin

Use

Tags

EGFP

Expression

Mutation

Q12H and deletion of F13 (please see depositor co…

Promoter

5UAS

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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XE244 Mouse Naked-1 CS2p+

Plasmid

#16728

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Mouse Naked-1 (Nkd1 Mouse)

Use

Xenopus expression

Tags

His and myc

Expression

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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