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p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Promoter

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1b

Plasmid

#176025

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET28(+)-NudC

Plasmid

#175022

Purpose

Purification of NudC enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

nudC

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cavin4a in pBluescript(SK+)

Plasmid

#126567

Purpose

For generating RNA probes for in situ hybridisation. Parton lab clone ISB

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1b

Plasmid

#176021

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4a-mCherry

Plasmid

#176010

Purpose

Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEHISSSO6206

Plasmid

#97065

Purpose

Expressing Sulfolobus solfataricus orf SSO6206 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO6206

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

Plasmid

#100597

Purpose

piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Inserts

CRISPR Cas9 fused with human Glucocorticoid Receptor
mCherry
rtTA-M2

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Codon-optimized for human codon usage

Promoter

Dox-inducible TetO promoter and Human EEF1A1 prom…

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

Plasmid

#100596

Purpose

piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Inserts

CRISPR Cas9 fused with human Glucocorticoid Receptor
mCherry
rtTA-M2

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Codon-optimized for human codon usage

Promoter

Dox-inducible TetO promoter and Human EEF1A1 prom…

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsE

Plasmid

#97019

Purpose

Expressing Pseudomonas aeruginosa pqsE protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Quinolone signal response protein

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPAFabH

Plasmid

#97018

Purpose

Expressing Pseudomonas aeruginosa FabH protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

3-Oxoacyl-[acyl-carrier-protein] synthase III

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM1 WPRE

Plasmid

#236233

Purpose

AAV expression of mouse STIM1 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Stim1 (Stim1 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-STIM1 WPRE

Plasmid

#236234

Purpose

AAV expression of mouse STIM1 internally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Stim1 (Stim1 Mouse)

Use

AAV

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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