We narrowed to 870 results for: Px330

1. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

   Plasmid

   #98971

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNA and spCas9
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREDIT_Cas9n-MS2-BB_BbsI

   Plasmid

   #164804

   Purpose

   REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HUWE1-sgRNA-1

   Plasmid

   #86924

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA1 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

6. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pYFP-NEAT1m_v1-RT

   Plasmid

   #97089

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1m_v1-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX-EGFP-g1

   Plasmid

   #107273

   Purpose

   eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP sgRNA-1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-GFP-SFPQ-RT

   Plasmid

   #97090

   Purpose

   Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   EGFP-SFPQ-RT (SFPQ Human)
   

   Use

   CRISPR

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human SOX17 sgRNA

    Plasmid

    #59725

    Purpose

    Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hSox17 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Human BLIMP1 sgRNA

    Plasmid

    #59724

    Purpose

    Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hBLIMP1 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pX-APRT-sg2

    Plasmid

    #107274

    Purpose

    APRT sgRNA-2 and Cas9 expression vector

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    APRT sgRNA-2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR/eCas9-GPIDAF

    Plasmid

    #213710

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR/eCas9-GPIBY55

    Plasmid

    #213709

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pML45

    Plasmid

    #206995

    Purpose

    Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

    Depositor

    Eugene Makeyev

    Article

    Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

    Insert

    Ptbp1 gRNA (Ptbp1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSF2 KO

    Plasmid

    #200208

    Purpose

    gRNA for HSF2 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF2 KO (Hsf2 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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