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pRK5-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194553

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

Plasmid

#194696

Purpose

HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

elavl3(HuC)

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pWPT-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49235

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mouse PKA-RIIalpha-mEGFP

Plasmid

#45527

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII alpha tagged by mEGFP

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mouse PKA-RIalpha-mEGFP

Plasmid

#45525

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RI alpha tagged by mEGFP

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-MUT

Plasmid

#194562

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(K42M)

Plasmid

#165440

Purpose

Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(T286A)

Plasmid

#165439

Purpose

Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(T286A)

Use

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

T286A: Autophosphorylation deficient mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mouse PKA-RIbeta-mEGFP

Plasmid

#45526

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RI beta tagged by mEGFP

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-rat PKA-RIIbeta-mEGFP

Plasmid

#45523

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-IDR

Plasmid

#194551

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-K184Rfs

Plasmid

#194544

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Bacterial

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mouse PKA-Calpha-mEGFP

Plasmid

#45528

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA catalytic subunit alpha tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Expression

Mutation

Promoter

actin15

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RtoK

Plasmid

#194559

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > K substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-KtoR

Plasmid

#194560

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K > R substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mEGFP-CaMKIIa (T305D/T306D)

Plasmid

#127611

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 305/306 to Alanine

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pB-6HIS-TEV-mEGFP,IRES-NGFR

Plasmid

#164521

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-rat PKA-RIIbeta-mEGFP

Plasmid

#45530

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge