We narrowed to 14,023 results for: egf

1. AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)

   Plasmid

   #181760

   Purpose

   targeting vector

   Depositor

   Tanya Daigle

   Insert

   nls-tagged EGFP and nls-tagged dTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132408

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLbaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R600A; H605A; R1243A; H1248A

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP

   Plasmid

   #228359

   Purpose

   RfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdown

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   2A-EGFP, 2A-mCD4, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

   Plasmid

   #191164

   Purpose

   Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP (additional cutsites)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)

   Plasmid

   #154470

   Purpose

   cDNA of NPM-ALK in the MSE backbone

   Depositor

   Cornelius Miething

   Article

   Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

   Insert

   NPM-ALK
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

   Plasmid

   #220508

   Purpose

   TGFb-inducible fluorescent reporter (TIE:EGFP)

   Depositor

   Leonard Zon

   Inserts

   TGFb inducible enhancer
   beta-globin minimal promoter
   SV40 PolyA signal
   

   Use

   Zebrafish expression (tol2)

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

   Plasmid

   #191159

   Purpose

   Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:EGFP.s2:mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132412

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dAdmCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R269A; H274A; R837A; H842A

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid

    #202198

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid

    #140004

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP1076-Ai213 (TICL-EGFP-ICF-mOrange2-ICN-mKate2)

    Plasmid

    #163491

    Purpose

    Conditional expression of 3 different fluorophores

    Depositor

    The Allen Institute for Brain Science, Tanya Daigle

    Insert

    EGFP, mOrange2, mKate2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135812

    Purpose

    This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    TagRFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

    Plasmid

    #191690

    Purpose

    For knocking p2a-EGFP into OTX2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    OTX2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163063

    Purpose

    Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Gateway entry vector

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

    Plasmid

    #196875

    Purpose

    Neuron-specific expression of LynGFP reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500

    Plasmid

    #45788

    Depositor

    Richard Murray

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD-tetO1

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

    Plasmid

    #191688

    Purpose

    For knocking p2a-EFGP into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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