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pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway Cloning

Tags

NLS

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLG173

Plasmid

#124368

Purpose

Control plasmid with only spectinomycin

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

empty backbone (control) (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

No promoter

Availability

Academic Institutions and Nonprofits only

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pBA707

Plasmid

#186238

Purpose

KanR-BBR1 entry vector for HR donor

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HP4_M13

Plasmid

#120340

Purpose

Helper plasmid for production of phagemid ssDNA, based on m13mp18

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13 phage genome

Expression

Bacterial

Promoter

native phage promoters

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Expression

Bacterial

Promoter

native phage promoters

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Hs.NRAS Q61R

Plasmid

#83179

Purpose

Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

Depositor

Dominic Esposito

Insert

NRAS (NRAS Human)

Use

Gateway Cloning

Mutation

Q61R

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR TERRA

Plasmid

#111383

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA

Use

Gateway entry cloning

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-FURIN

Plasmid

#158442

Purpose

Gateway Cloning compatible entry vector for the human FURIN gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Gateway Cloning

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti spCas9 T2A iRFP670 P2A puro

Plasmid

#122182

Purpose

The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

Depositor

Raphael Gaudin

Inserts

spCas9 (NEWENTRY )
iRFP670

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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