Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,118 results for: gibson assembly

Clear keywords
Showing: 701 - 720 of 1118 results

  2. pBN215

    Plasmid
    #167962
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Publication
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167962-plasmid-map-sequence-id-344789

    Enlarge

  3. pBN261

    Plasmid
    #167966
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Publication
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167966-plasmid-map-sequence-id-344799

    Enlarge

  6. pBN083

    Plasmid
    #173445
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Depositor
    James Naismith
    Publication
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y705E, Y706E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173445-plasmid-map-sequence-id-344777

    Enlarge

  9. pBN008

    Plasmid
    #167949
    Purpose
    express E. coli E69 (O9a:K30) Wzc with K540M mutation
    Depositor
    James Naismith
    Publication
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167949-plasmid-map-sequence-id-344798

    Enlarge

  20. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Publication
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

    Enlarge

Showing: 701 - 720 of 1118 results