A plasmid-based Escherichia coli gene expression system with cell-to-cell variation below the extrinsic noise limit.
Hensel Z
PLoS One. 2017 Oct 30;12(10):e0187259. doi: 10.1371/journal.pone.0187259. eCollection 2017. PubMed
Hensel Z
PLoS One. 2017 Oct 30;12(10):e0187259. doi: 10.1371/journal.pone.0187259. eCollection 2017. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102664 | pZH509 | Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102665 | pZH511 | Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102666 | pZH512 | Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102667 | pZH514 | Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102669 | pZH516 | Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102670 | pZH517 | Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102671 | pZH518 | Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102672 | pZH519 | Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102673 | pZH520 | Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102674 | pZH521 | Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |
| 102675 | pZH522 | Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly |