We narrowed to 757 results for: mRuby

1. pcDNA3.1(+)-FluoSTEP-AKAR

   Plasmid

   #181844

   Purpose

   Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-AKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ARB366

   Plasmid

   #124049

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ARB367

   Plasmid

   #124050

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-mR2-ANLN_sgRNA

   Plasmid

   #183876

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-mR2-RHOA_sgRNA

   Plasmid

   #183877

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_ACTB CRISPIE

   Plasmid

   #172853

   Purpose

   Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Inserts

   Actb intron 1 sgRNA
   DRS-2 sgRNA
   CRISPIE designer exon (phase 0-0) encoding mEGFP
   mRuby3
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-mR2-ACTB_sgRNA

   Plasmid

   #183884

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIA3

    Plasmid

    #109399

    Purpose

    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB

    Depositor

    Roger Foo

    Article

    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby2 and mtp53dn

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ad:2CMV_A2R2-N25Ctr

    Plasmid

    #122243

    Purpose

    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

    Promoter

    CMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS1575

    Plasmid

    #239523

    Purpose

    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2

    Depositor

    John Dueber

    Insert

    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKS1613

    Plasmid

    #239531

    Purpose

    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    mTurquoise2-CpPT1(52-408)-SKL
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKS1574

    Plasmid

    #239522

    Purpose

    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2

    Depositor

    John Dueber

    Insert

    ScPex22(1-36)-ScYea4p-mTurquoise2
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVL1337

    Plasmid

    #239541

    Purpose

    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-ScPex15(315-383)
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS1612

    Plasmid

    #239530

    Purpose

    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CpPT1(52-408)-SKL
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVL1279

    Plasmid

    #239538

    Purpose

    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2

    Depositor

    John Dueber

    Insert

    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJB1338

    Plasmid

    #218592

    Purpose

    Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex22
    

    Expression

    Yeast

    Mutation

    Pex22(1-36)

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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