We narrowed to 15,952 results for: grna

1. pICSL4723_ HDT701

   Plasmid

   #126886

   Purpose

   Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTRY4_Rac5

   Plasmid

   #126897

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTRY4_ OBfold

   Plasmid

   #126901

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL4723_ OBfold

   Plasmid

   #126889

   Purpose

   Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTRY4_ HDT701

   Plasmid

   #126898

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTRY4_ ERF922

   Plasmid

   #126893

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC38

   Plasmid

   #104812

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Nevin Young

   Insert

   Medtr3g107390
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASCADE-zwf

   Plasmid

   #65825

   Purpose

   zwf targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   zwf guide
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induc…

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASCADE-udhA

   Plasmid

   #65818

   Purpose

   udhA targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   udhA guide
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induc…

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCASCADE-gltA2

    Plasmid

    #65817

    Purpose

    gltA targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA2 guide
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

    Plasmid

    #84250

    Purpose

    Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRISPR_HLA_class_I_2

    Plasmid

    #164987

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

    Plasmid

    #84248

    Purpose

    Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    Alejandro Chavez, George Church

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Promoter

    S.pombe rrk1 and nmt41

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPR_TRAC

    Plasmid

    #164993

    Purpose

    Expression of gRNA targeting TCRalpha constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRAC
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH75

    Plasmid

    #128224

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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