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K420CLM RP

Plasmid

#24443

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-281&390-428.L239R

Plasmid

#15959

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; L239R mutation

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (B) Eya2 (CT#665)

Plasmid

#13877

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Eya2 (EYA2 Chicken)

Use

Retroviral; Avian expression

Mutation

*

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
CLM RP KGF Mut 1

Plasmid

#24166

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
HOXB4-NA-MSCV

Plasmid

#20987

Depositor

Corey Largman

Article

Krosl et al Oncogene. 1998 Jul 2. 16(26):3403-12.

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N51A (makes DNA binding mutant protein)

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
RCAS (A) Lunatic Fringe (CT#482)

Plasmid

#13977

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pUC19_CoV-2_frC

Plasmid

#253184

Purpose

For recombinant virus rescue; encodes fragment C of SARS-CoV-2 (nt15100-20958)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frC

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_SV40

Plasmid

#253185

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD1

Plasmid

#253115

Purpose

Encodes SARS-CoV-2 Wuhan spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Wuhan Spike

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frB

Plasmid

#253183

Purpose

For recombinant virus rescue; encodes fragment B of SARS-CoV-2 (nt8590-15107)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frB

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF68

Plasmid

#253129

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf6 and orf8

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF68

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-HsFBXO28-Myc-MBP-HA

Plasmid

#228945

Purpose

Expression HsFBXO28-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

HsFBXO28 (FBXO28 Human)

Tags

myc

Expression

Bacterial

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i-Sec61β

Plasmid

#250997

Purpose

Expression of Sec61β with an N-terminal mScarlet tag

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCSCG-Fc-pST3Gal1 sCore2

Plasmid

#228822

Purpose

Expresses Fc-fusion pST3Gal1 sCore2 (H302A/A312I/F313S) in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 sCore2

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted and H30…

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTomato)

Plasmid

#248358

Purpose

Lentiviral vector that can express FKBP12 along with N terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

3xFLAG-NES-FKBP12-(G4S)4x-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CpG-free-mStayGold

Plasmid

#241386

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CpG-free

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-Fc-pST3Gal1 Dead

Plasmid

#228821

Purpose

Expresses Fc-fusion pST3Gal1 Dead (Q108A/Y233A/Y269F) in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 Dead

Use

Lentiviral

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted and Q10…

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pT7RNAPNev-NB1

Plasmid

#216336

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

Depositor

Mark Styczynski

Article

McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

Insert

T7RNAPN-NB1 fusion

Expression

Bacterial

Available Since

July 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a

Plasmid

#233461

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a1

Plasmid

#233462

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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