-
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET26b-Granzyme A (S-A)
Plasmid#8825DepositorInsertHuman Granzyme A inactive (GZMA Human)
UseTags(His)6ExpressionBacterialMutationchanged Serine 184 to AlaninePromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET_RFP-SZ2
Plasmid#124933PurposeExpression of RFP-SZ2DepositorInsertRFP-SYNZIP2
UseTagsHis and mCherryExpressionBacterialMutationSZ2 domainPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 661-1014PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneUseTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGlnA-Ypet
Plasmid#98278Purposemammalian expression of GlnA-YpetDepositorInsertGlnA
UseTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR
Plasmid#173940PurposeBacterial expression of PARP1 lacking WGR domainDepositorInsertPARP1delWGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET30B-PTEN
Plasmid#20741DepositorInsertPTEN (PTEN Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertBetacellulin (BTC Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-BoNT/E
Plasmid#168020PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cellsDepositorInsertBoNT/E-LC(1-411)-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN_FL
Plasmid#30223DepositorInsertrhVitronectin without deletions (VTN Human)
UseTagsExpressionBacterialMutationNo deletionsPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only