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pLV_TRET_hNgn1_UBC_Bla

Plasmid

#61473

Purpose

3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn1 (NEUROG1 Human)
Bla

Use

Lentiviral

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B4-HA

Plasmid

#209212

Purpose

Mammalian expression of ATP8B4

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B4 (ATP8B4 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Tags

Flag

Expression

Mammalian

Mutation

*

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2

Plasmid

#85208

Purpose

Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Marta Gallego, Lothar Hennighausen

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNAI-GAL4-CREB

Plasmid

#46769

Purpose

Expression of GAL4 fusion to wild type CREB

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp16

Plasmid

#157723

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10B-HA

Plasmid

#209216

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10B (ATP10B Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B2-HA

Plasmid

#209210

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B2 (ATP8B2 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MDV1A

Plasmid

#196679

Purpose

Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RSVGSVY insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS

Plasmid

#175006

Purpose

Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-dDIO-vCre

Plasmid

#118029

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-dDIO-vCre

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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