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PresentER-NLVPMVATV (GFP)

Plasmid

#102947

Purpose

PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

Depositor

David Scheinberg

Article

Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

Insert

NLVPMVATV

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_SEPT9 i1

Plasmid

#71609

Purpose

mammalian expression of human SEPT9 i1 with a GFP fusion.

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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CD40L-MIEG-hCD4 (hCD4-CD40L)

Plasmid

#40355

Depositor

Alexander Dent

Article

Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

Insert

CD40L (Cd40lg Mouse)

Use

Retroviral

Tags

IRES hCD4

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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LLP775_αGCN4-p65HSF1-CO

Plasmid

#211768

Purpose

SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-p65HSF1

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S-AtUBA1-S

Plasmid

#222714

Purpose

Expression AtUBA1-S and AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtUBC8 (UBC8 Mustard Weed)
ATUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM

Plasmid

#169982

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX305-N-dTAG-CARD8

Plasmid

#169989

Purpose

Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

dTAG

Expression

Mammalian

Promoter

hPGK

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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mRFP-mem

Plasmid

#55622

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

mRFP-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTrcHisB-TRF1

Plasmid

#53209

Purpose

Expressed N-terminally Hexahistidine tagged TRF1 protein

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF1 (TERF1 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

3-439

Promoter

Trc

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3B WT

Plasmid

#123072

Purpose

Gateway entry clone encoding human MAP1LC3B wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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H9 pMalLP

Plasmid

#41811

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

H9 Super-2 (IL2 Human)

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Promoter

pTac

Available Since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Article

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2(apf)

Plasmid

#13761

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2(apf) (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif THr(183)-Pro-T…

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Expression

Mammalian

Mutation

aa 268-476

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only