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pRP.CEP290 c.2991+1655A>G

Plasmid

#226556

Purpose

Minigene encoding the CEP290 c.2991+1655A>G mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

CEP290 (CEP290 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

c.2991+1655A>G intronic mutation

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapERK)d1EGFP (KroxDs)

Plasmid

#214912

Purpose

for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

d1EGFP under the MapERK promoter

Expression

Mammalian

Promoter

MapkERK promoter

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRP.ABCA4 c.5461-10T

Plasmid

#226553

Purpose

Minigene encoding the ABCA4 c.5461-10T mutation

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

ABCA4 (ABCA4 Human)

Expression

Mammalian

Mutation

c.5461-10T intronic mutation

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb5-flag

Plasmid

#86769

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 5 (PSMB5 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2_2A_Sox11-PURO

Plasmid

#226284

Purpose

Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)

Use

Lentiviral

Tags

NGN2 and SOX11 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-hADAR2dd-pAG

Plasmid

#248101

Purpose

Expresses rAPOBEC1-hADAR2dd-pAG in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
hADAR2dd (ADARB1 Human)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC39A11_STOP

Plasmid

#161307

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A11 (SLC39A11 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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actc1b-GcAMP5

Plasmid

#126563

Purpose

Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B

Plasmid

#180380

Purpose

lentiviral transduction of human ZBTB7B gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyeA3A-BE4max

Plasmid

#157946

Purpose

Lentiviral expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti -117G-hyeA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N57G

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pME-GcAMP5

Plasmid

#126569

Purpose

Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Use

Gateway Cloning

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb2-flag

Plasmid

#86766

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 2 (PSMB2 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Irx4

Plasmid

#72692

Purpose

Doxycyline regulated expression of Irx4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Irx 4 (IRX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nAChr-beta3-YFP

Plasmid

#50484

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)

Tags

YFP

Expression

Mammalian

Mutation

YFP fusion in M3-M4 loop (after residue P379). O…

Promoter

CMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only