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pQdCas12a.sggfp(B)

Plasmid

#236040

Purpose

The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Publication

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13198

Plasmid

#219873

Purpose

The base plasmid of TUNEYALI forTF08

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08

Expression

Yeast

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG006

Plasmid

#231310

Purpose

Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

Depositor

Barney Geddes

Publication

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

Expression

Bacterial

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A1.2-GFP (pc2189)

Plasmid

#223708

Purpose

Expression of macroH2A1.2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Publication

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.2-GFP (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Publication

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Cre

Use

AAV

Promoter

cTnT

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

Plasmid

#223163

Purpose

2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13220

Plasmid

#219895

Purpose

The base plasmid of TUNEYALI for TF30

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30

Expression

Yeast

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-Quartet

Plasmid

#214727

Purpose

In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusion

Depositor

Mark Howarth

Publication

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

SpyTag-Quartet (S Synthetic)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13200

Plasmid

#219875

Purpose

The base plasmid of TUNEYALI forTF10

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13245

Plasmid

#219916

Purpose

The base plasmid of TUNEYALI for TF55

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55

Expression

Yeast

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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