Addgene

We narrowed to 4,047 results for: ALK

Showing: 761 - 780 of 4047 results

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pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

Plasmid

#85430

Purpose

[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10 T2A PD-L1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry

Plasmid

#214143

Purpose

Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

Plasmid

#85427

Purpose

Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.41

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ39 - Peft-3 Cre (PATCs)

Plasmid

#191381

Purpose

High efficiency CRE recombinase expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Peft-3 Cre tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Peft-3

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GCaMP-AMPK-SPARK

Plasmid

#226257

Purpose

Dual reporter of Ca2+ levels and AMPK activity

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

GCaMP-AMPK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-Cas9-2A-EGFP

Plasmid

#167928

Purpose

Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

T2A-EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tTRE, hPGK

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-mCherry

Plasmid

#20938

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Tags

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pOT_7-lenti-EFS-SS1-BBz-2A-puro

Plasmid

#217990

Purpose

Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Xue et al bioRxiv 2024.11.27.625752

Insert

SS1-BBz CAR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85433

Purpose

Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

leucine zipper human TRAIL secreted form

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

Plasmid

#85424

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-7_synNotch

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85432

Purpose

Lentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human TRAIL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only
pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
Ppst93-ANME2bHR1 MCR-NG

Plasmid

#192774

Purpose

Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-ANME1G37 MCR-NG

Plasmid

#192773

Purpose

Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYSD085

Plasmid

#212922

Purpose

Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

649 Stalk

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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P70a-deGFP-sc101-kan

Plasmid

#184841

Purpose

Expression of deGFP

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

promoter OR2-OR1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-2_T2A_MIP1α_PGK_mCherry

Plasmid

#85429

Purpose

Lentiviral vector for Gal4 inducible human IL-2/MIP1a with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Human IL-2 T2A MIPalpha

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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