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pCMV5-Flag-EPLIN beta

Plasmid

#40929

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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HP35 tension sensor

Plasmid

#101250

Purpose

Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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B2345_BTB2x-mCherry

Plasmid

#215097

Purpose

Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB-BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

Plasmid

#89870

Purpose

expresses DRD2-iTango component in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-dCas13b-ADAR2DD

Plasmid

#190913

Purpose

Addgene #103871 with 3x FLAG on N-terminal of dCas13

Depositor

Timothy Jarome

Article

Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

Insert

3X FLAG

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-11A55B [S218E/S222D]

Plasmid

#40809

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

S218E, S222D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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HRAS

Plasmid

#55653

Purpose

expression of mouse HRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

HRAS (Hras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pLHCX-FLAG-mPKM1

Plasmid

#44240

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M1 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

Plasmid

#183150

Purpose

Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Inserts

Lex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD

Use

For yeast genomic integration

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-Optimized E6AP

Plasmid

#165100

Purpose

ecoli optimized expression of GST-fusion of human E6AP

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

Insert

E6AP isoform 3 (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRST7-ER beta (short)

Plasmid

#11357

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ER beta short (ESR2 Human)

Expression

Mammalian

Available Since

March 10, 2006

Availability

Academic Institutions and Nonprofits only