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Dcp2 ΔH1 WD

Plasmid

#163580

Purpose

express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

W50A, D54A, L255A

Promoter

DCP2 promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H-H1

Plasmid

#163584

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

Promoter

DCP2 promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300 AAAA

Plasmid

#163579

Purpose

express Dcp2 300 AAAA at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domain (301-970); mutati…

Promoter

DCP2 promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1

Plasmid

#163576

Purpose

express Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Lysine at position 255 is mutated to Alanine

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300 ΔH1

Plasmid

#163578

Purpose

express Dcp2 300 ΔH1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domain (301-970); Lysine…

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL-H1

Plasmid

#163583

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

The first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-C4A,C5A,C7A-GFP

Plasmid

#166838

Purpose

Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

C4A, C5A, C7A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pVG97

Plasmid

#164849

Purpose

Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA

Expression

Yeast

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pVG50

Plasmid

#164708

Purpose

yEVenus under the control of tetOpr with ADH1t upstream of the reporter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFVGe2

Plasmid

#149500

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLlCa5

Plasmid

#149501

Purpose

Broad-Host range expression vector, pBBR origin, LacI repressor, PLlacO-1 promoter, AmpR, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-AmpR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKPcR5

Plasmid

#149467

Purpose

Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKVR5

Plasmid

#149469

Purpose

Broad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔN

Plasmid

#163575

Purpose

express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

Depositor

Roy Parker

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of N-terminal domain (1-242) of Dcp2

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1245

Plasmid

#155139

Purpose

Expresses MlaF in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaF

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1246

Plasmid

#155141

Purpose

Expresses MlaB in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaB

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKP920

Plasmid

#160814

Purpose

Expression of ExbB protein

Depositor

Kathleen Postle

Publication

Ollis et al J Bacteriol. 2012 Jun;194(12):3069-77. d

Insert

exbB

Expression

Bacterial

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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