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Addgene
Showing: 61 - 80 of 399 results
  1. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Tags
    Expression
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Availability
    Academic Institutions and Nonprofits only
  2. pST50Trc1-HISDHFR

    Plasmid
    #63935
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. pST50Trc1-CBPDHFR

    Plasmid
    #63936
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. pST50Trc1-STRDHFR

    Plasmid
    #63937
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  5. pST50Trc1-HPCDHFR

    Plasmid
    #63939
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  6. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. pST50Trc1-CBPNDHFR

    Plasmid
    #63941
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Calmodulin Binding Peptide (CBP); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. pST50Trc1-STRNDHFR

    Plasmid
    #63942
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. pST50Trc1-FLAGNDHFR

    Plasmid
    #63943
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  10. pST50Trc1-HPCNDHFR

    Plasmid
    #63944
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 80 of 399 results