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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 78 results for: FreeGenes

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Showing: 61 - 78 of 78 results

  1. pOpen-Pfulig

    Plasmid
    #165525
    Purpose
    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pfu DNA Ligase
    Use
    Synthetic Biology
    Available since
    Aug. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165525-plasmid-map-sequence-id-338923

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  2. pOpen-dbh

    Plasmid
    #165513
    Purpose
    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Sulfolobus DNA Polymerase IV
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165513-plasmid-map-sequence-id-338598

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  4. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  5. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

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  6. pOpen-Fpg

    Plasmid
    #165541
    Purpose
    Formamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Fpg
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165541-plasmid-map-sequence-id-339267

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  7. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.PstI
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  8. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  16. pOpen-T4-BGT

    Plasmid
    #165530
    Purpose
    T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4-BGT
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165530-plasmid-map-sequence-id-339085

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  17. pOpen-HSlig4

    Plasmid
    #165503
    Purpose
    Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase IV
    Use
    Synthetic Biology
    Available since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165503-plasmid-map-sequence-id-338457

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  18. pOpen-ECOligA

    Plasmid
    #165564
    Purpose
    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Ligase
    Use
    Synthetic Biology
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165564-plasmid-map-sequence-id-340043

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Showing: 61 - 78 of 78 results