We narrowed to 82 results for: Neisseria

1. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47870

   Purpose

   All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   OCT4 crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. CNC3 (CASANOVA-C3)

   Plasmid

   #137191

   Purpose

   Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

   Insert

   CNC3 (CASANOVA-C3)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_HyPer7RgDAAO

   Plasmid

   #217746

   Purpose

   Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47868

   Purpose

   This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. CNC3G (CASANOVA-C3 with glycine linkers)

   Plasmid

   #137192

   Purpose

   Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

   Insert

   CNC3G (CASANOVA-C3 with glycine linkers)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP

   Plasmid

   #226113

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-1-5p Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

   Plasmid

   #226114

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

   Plasmid

   #226115

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-107 Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

   Plasmid

   #226109

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-92a Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

    Plasmid

    #226110

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-378a Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP

    Plasmid

    #226111

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-22 Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

    Plasmid

    #226112

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-33b Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA303

    Plasmid

    #216071

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid

    #61365

    Purpose

    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    Nm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D16A, D587A, H588A, N611A in Nm Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Use

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-A1IQ68

    Plasmid

    #103080

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A1IQ68(Cas9 coding gene from Neisseria meningitidis serogroup A / serotype 4A (strain Z2491))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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