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We narrowed to 98 results for: Neisseria

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Showing: 61 - 80 of 98 results

  2. NmMetQ C20A

    Plasmid
    #172112
    Purpose
    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine on MetQ and Cystein…
    Promoter
    T7 Promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172112-plasmid-map-sequence-id-344822

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  3. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid
    #246438
    Purpose
    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    NmeCas9
    Nme-sgRNA
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 16 to Alanine, deleted amin…
    Promoter
    EF-1 alpha and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246438-plasmid-map-sequence-id-490930

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  4. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid
    #228735
    Purpose
    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Insert
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228735-plasmid-map-sequence-id-453996

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  9. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available Since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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  10. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217746-plasmid-map-sequence-id-442633

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  13. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240513-plasmid-map-sequence-id-477190

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  14. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

    Plasmid
    #240514
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-20 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240514-plasmid-map-sequence-id-477194

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  15. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid
    #240515
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-125a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240515-plasmid-map-sequence-id-481869

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  16. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid
    #240516
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-151a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240516-plasmid-map-sequence-id-477188

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  17. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid
    #240517
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-221 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240517-plasmid-map-sequence-id-477189

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  19. pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP

    Plasmid
    #226113
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-1-5p Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226113-plasmid-map-sequence-id-448319

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  20. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #226114
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226114-plasmid-map-sequence-id-448323

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Showing: 61 - 80 of 98 results